Recombinant human CD14 protein

CD14, also known as monocyte differentiation antigen CD14, is a member of the eucine-rich repeat (LRR) proteins family. It exists in two forms, one anchored to the membrane by a GPI-tail (mCD14), the other a soluble form (sCD14) . It is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide (LPS) . It acts as a co-receptor (along with TLR4 and MD-2) and helps to detect bacteria in the body by binding LPS, a pathogen-associated molecular pattern (PAMP) . Recombinant human CD14, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD14 enhance the IL-8 secretion of the THP‑1 human acute monocytic leukemia cells in the presence of LPS. The ED50 range ≤ 100 ng/mL.

Product Name Alternative

Monocyte differentiation antigen CD14, CD14 molecule, Myeloid cell-specific leucine-rich glycoprotein

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its ability to enhance IL-8 secretion using THP‑1 human acute monocytic leukemia cells in the presence of LPS. The ED50 range ≤ 100 ng/mL.

Molecular Weight

35.8kDa (331aa)

Additionnal Information

ATGP4095-10 µg, ATGP4095-20 µg, ATGP4095-50 µg, ATGP4095-100 µg, ATGP4095-250 µg, ATGP4095-500 µg, ATGP4095-1 mg, ATGP4095-10, ATGP4095-20, ATGP4095-50, ATGP4095-100, ATGP4095-250, ATGP4095-500, ATGP4095-1