Recombinant human IgG1 Fc protein

Immunoglobulins are membrane-bound or secreted glycoproteins produced by B lymphocytes. IgG antibodies protect the body against the pathogens by agglutination and immobilization, complement activation, toxin neutralization, as well as the antibody-dependent cell-mediated cytotoxicity. IgG1 is most abundant in serum among the four IgG subclasses (IgG1, 2, 3 and 4) and binds to Fc receptors on phagocytic cells with high affinity. IgG comprise two identical light (L) and heavy (H) chains forming a ‘Y‐shaped’ structure. It also is digested by pepsin proteolysis into two distinct fragments (Fab, Fc) . Fc fragment is a homodimeric glycoprotein with one monomer consisting of two constant immunoglobulin domains (CH2, CH3) from the C‐terminal half of one heavy chain. Through interaction with its ligands (FcγR, C1q and FcRn) it mediates various immune effector functions and increases the half‐life of the antibody molecule. Recombinant IgG Fc is suggested to represent a potential anti-inflammatory drug for treatment of human autoimmune diseases. Recombinant human IgG1 Fc was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human Fc gamma RIIIA/CD16a (CAT# ATGP4102) is coated at 10 µg/mL (100 ul/well) can bind Human IgG1 Fc. The ED50 range ≤ 1.5 µg/mL.Human IgG1 Fc is coated at 1 µg/mL (100 ul/well) can bind Human Fc gamma RIIA/CD32a (H167) (CAT# ATGP4116) in a Functional ELISA assay.{2208220001306487.jpg}Human Fc gamma RIIIA/CD16a (CAT# ATGP4098) is coated at 10 µg/mL (100 ul/well) can bind Human IgG1 Fc. The ED50 range ≤ 4 µg/mL.{2208220001402774.jpg}

Product Name Alternative

Immunoglobulin heavy chain constant region gamma 1, IGHG1, Ig gamma-1 chain C region, Ig gamma-1 chain C region EU, Ig gamma-1 chain C region KOL, Ig gamma-1 chain C region NIE

Expression System

HEK293

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by BCA assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

1. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4102) . The ED50 range ≤ 1.5 µg/mL.; ; 2. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4116) .; ; 3. Measured by its binding ability in a functional ELISA with Human Fc gamma RIIIA/CD16a (CAT# ATGP4098) . The ED50 range ≤ 4 µg/mL.

Molecular Weight

25.9kDa (231aa)

Additionnal Information

ATGP4094-10 µg, ATGP4094-20 µg, ATGP4094-50 µg, ATGP4094-100 µg, ATGP4094-250 µg, ATGP4094-500 µg, ATGP4094-1 mg, ATGP4094-10, ATGP4094-20, ATGP4094-50, ATGP4094-100, ATGP4094-250, ATGP4094-500, ATGP4094-1

References & Citations

Michael McHeyzer-Williams., et al, (2012) Nat. Rev. Immunol. 12:24-34.; ; Schroeder H.W. Jr., et al, (2010) J. Allergy Clin. Immunol. 125:S41-S52.; ; E D Carosella., et al, (1988) Cell Immunol. 112:262-270.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

CAC20454.1

Uniprot Accession Number

P01857

Species

Human

N Terminal Sequence

Pro 100

AA Sequence

PKSCDKTHTC PPCPAPELLG GPSVFLFPPK PKDTLMISRT PEVTCVVVDV SHEDPEVKFN WYVDGVEVHN AKTKPREEQY NSTYRVVSVL TVLHQDWLNG KEYKCKVSNK ALPAPIEKTI SKAKGQPREP QVYTLPPSRD ELTKNQVSLT CLVKGFYPSD IAVEWESNGQ PENNYKTTPP VLDSDGSFFL YSKLTVDKSR WQQGNVFSCS VMHEALHNHY TQKSLSLSPG K