Recombinant mouse Cathepsin C/DPPI protein
Cathepsin C/DPPI, also known as dipeptidyl peptidase I , is a lysosomal exo-cysteine protease belonging to the peptidase C1 protein family, a subgroup of the cysteine cathepsins. It catalyses excision of dipeptides from the N-terminus of protein and peptide substrates, except if the amino group of the N-terminus is blocked. Also, It appears to be a central coordinator for activation of many serine proteases in immune/inflammatory cells. Defects in the Cathepsin C have been shown to be a cause of Papillon-Lefevre disease, an autosomal recessive disorder characterized by palmoplantar keratosis and periodontitis. Also, It plays a key role in the activation of several degradative enzymes linked to tissue destruction in inflammatory diseases. Recombinant mouse Cathepsin C, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 50,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Gly-Arg-AMC per minute at pH 6.0 at 37C.
Product Name Alternative
Cathepsin C, AI047818, CatC, DP, DPP1, DPPI, Cathepsin J, Dipeptidyl peptidase isoform 1, Dipeptidyl peptidase 1, Dipeptidyl transferase, CTSC
Expression System
HEK293
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Specific activity is > 50,000 pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of Gly-Arg-AMC per minute at pH 6.0 at 37C.
Molecular Weight
50.5kDa (444aa)
Additionnal Information
References & Citations
Dahl SW., et al, (2001) Biochemistry. 40:1671-1678. ; ; Turk D., et al, (2001) The EMBO Journal. 20:6570-6582.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Enzymes & Proteases
NCBI Accession Number
NP_034112.3
Uniprot Accession Number
P97821
Species
Mouse
N Terminal Sequence
Asp25
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