Recombinant human PRCP protein

PRCP, also known as prolylcarboxypeptidase, is a member of the peptidase S28 family of serine exopeptidases. This protein is proteolytically processed to generate the mature lysosomal prolylcarboxypeptidase. This enzyme cleaves C-terminal amino acids linked to proline in peptides such as angiotension 2, 3 and des-Arg9-bradykinin. The cleavage occurs at acidic pH, but the enzyme activity is retained with some substrates at neutral pH. It has been shown to be an activator of the cell matrix-associated prekallikrein. The importance of angiotension 2, one of the substrates of this enzyme, in regulating blood pressure and electrolyte balance suggests that PRCP may be related to essential hypertension. Recombinant human PRCP, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 3,000 pmol/min/μg, and is defined as the amount of enzyme that convert 1.0 pmole of Z-Pro-Ala-OH per minute at pH 4.0 at 25C.

Product Name Alternative

Prolylcarboxypeptidase, HUMPCP, PCP, lysosomal Pro-X carboxypeptidase isoform1, Angiotensinase C, Lysosomal carboxypeptidase C, Proline carboxypeptidase

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Specific activity is > 3,000 pmol/min/μg, and is defined as the amount of enzyme that convert 1.0 pmole of Z-Pro-Ala-OH per minute at pH 4.0 at 25C.

Molecular Weight

54.3kDa (481aa)

Additionnal Information

ATPG4088-10 µg, ATPG4088-20 µg, ATPG4088-50 µg, ATPG4088-100 µg, ATPG4088-250 µg, ATPG4088-500 µg, ATPG4088-1 mg, ATPG4088-10, ATPG4088-20, ATPG4088-50, ATPG4088-100, ATPG4088-250, ATPG4088-500, ATPG4088-1