Recombinant rat TrkA protein

TrkA, also known as Tyrosine kinase receptor A, is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. It leads to cell differentiation and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. In one study conducted on two rat models, an inhibition of TrkA with AR786 led to a reduction in joint swelling, joint damage, and pain caused by inflammatory arthritis. Recombinant rat TrkA, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Rat Ntrk1 in inhibit the cell growth using TF-1 human erythroleukemic cells in the presence of 0.5 ng/mL of rat NGF. The ED50 range ≤ 5 ng/mL.

Product Name Alternative

High affinity nerve growth factor receptor, Neurotrophic tyrosine kinase receptor type 1, NTRK-1, NTRK1, Slow nerve growth factor receptor, p140-TrkA, Trk-A, Trk, Trka, TRK1-transforming tyrosine kinase protein, TRKAOncogene TRK, TRKTRK1, tyrosine kinase receptor A

Expression System

HEK293

Antigen Species

Rat

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Measured by ability to inhibit NGF-induced proliferation assay using TF‑1 human erythroleukemic cells in the presence of 0.5 ng/mL of rat NGF. The ED50 range ≤ 5 ng/mL.

Molecular Weight

69kDa (623aa)

Additionnal Information

ATGP4085-10 µg, ATGP4085-20 µg, ATGP4085-50 µg, ATGP4085-100 µg, ATGP4085-250 µg, ATGP4085-500 µg, ATGP4085-1 mg, ATGP4085-10, ATGP4085-20, ATGP4085-50, ATGP4085-100, ATGP4085-250, ATGP4085-500, ATGP4085-1