Recombinant rat TrkA protein
TrkA, also known as Tyrosine kinase receptor A, is a member of the neurotrophic tyrosine kinase receptor (NTKR) family. This kinase is a membrane-bound receptor that, upon neurotrophin binding, phosphorylates itself and members of the MAPK pathway. It leads to cell differentiation and may play a role in specifying sensory neuron subtypes. It has a crucial role in the development and function of the nociceptive reception system as well as the establishment of thermal regulation via sweating. In one study conducted on two rat models, an inhibition of TrkA with AR786 led to a reduction in joint swelling, joint damage, and pain caused by inflammatory arthritis. Recombinant rat TrkA, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Rat Ntrk1 in inhibit the cell growth using TF-1 human erythroleukemic cells in the presence of 0.5 ng/mL of rat NGF. The ED50 range ≤ 5 ng/mL.
Product Name Alternative
Expression System
HEK293
Antigen Species
Rat
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS - PAGE
Bioactivity
Molecular Weight
69kDa (623aa)
Additionnal Information
References & Citations
Benito-Gutiérrez E, et al. (2006) . Mol Cell Neurosci. 31:179-192.; ; Lambiase A, et al. (2005) . Natl Acad Sci. 102:16795-16800.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol.
Scientific Category
Neuroscience
NCBI Accession Number
NP_067600.1
Uniprot Accession Number
P35739
Species
Rat
N Terminal Sequence
Ser 35
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