Recombinant human TL1A/TNFSF15 protein

Tumor necrosis factor ligand superfamily member 15, also known TNFSF15, belongs to the TNF superfamily. TNFSF15 is predominantly expressed in endothelial cells and its expression is inducible by TNFalpha and IL1alpha. TNFSF15 is an angiogenesis inhibitor of the TNF family and functions in part by directly inhibiting endothelial cell proliferation. TNFSF15 may act as an autocrine factor to induce apoptosis in endothelial cells via activation of multiple signaling pathways, including stress protein kinases and certain caspases. Recombinant human TNFSF15 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human TNFSF15 induces apoptosis in TF-1 human erythroleukemic cells in the presence of cycloheximaide. The ED50 range ≤ 100 ng/mL.

Product Name Alternative

TNF superfamily member 15, TNF ligand-related molecule 1, TL1, Vascular endothelial cell growth inhibitor, VEGI, TNF superfamily ligand TL1A, TL1A, Vascular endothelial growth inhibitor-192A, VEGI192A

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its ability to induce apoptosis in TF-1 human erythroleukemic cells in the presence of cycloheximaide. The ED50 range ≤ 100 ng/mL.

Molecular Weight

22.7 kDa (201aa) confirmed by MALDI-TOF

Additionnal Information

TL1A, TNFSF15, Tumor necrosis factor ligand superfamily member 15, TL1, TL1A, VEGI, VEGI192A, ATGP4084-10 µg, ATGP4084-20 µg, ATGP4084-50 µg, ATGP4084-100 µg, ATGP4084-250 µg, ATGP4084-500 µg, ATGP4084-1 mg, ATGP4084-10, ATGP4084-20, ATGP4084-50, ATGP4084-100, ATGP4084-250, ATGP4084-500, ATGP4084-1

References & Citations

Migone T S., et al. (2002) Immunity. 16:479.; ; Zhai Y., et al. (1999) FASEB J.13:181-189.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 0.15M NaCl, 40% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_005109.2

Uniprot Accession Number

O95150

Species

Human

AA Sequence

LKGQEFAPS HQQVYAPLRA DGDKPRAHLT VVRQTPTQHF KNQFPALHWE HELGLAFTKN RMNYTNKFLL IPESGDYFIY SQVTFRGMTS ECSEIRQAGR PNKPDSITVV ITKVTDSYPE PTQLLMGTKS VCEVGSNWFQ PIYLGAMFSL QEGDKLMVNV SDISLVDYTK EDKTFFGAFL L