Recombinant human PKC iota protein
PKC iota, also known as Protein Kinase C iota, is a member of serine/threonine protein kinases family. It play a key regulatory role in a number of cellular functions including cell growth and differentiation, hormone secretion, and gene expression. It is involved in cell polarization processes and the formation of epithelial tight junctions. This protein kinase is calcium-independent and phospholipid-dependent. It is also implicated in the activation of several signaling pathways including Ras, c-Src and NF-kappa-B pathways. Recombinant human PKC iota, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 80% by SDS - PAGE
Molecular Weight
69kDa (602aa)
Additionnal Information
References & Citations
Dey A et al, (2021) Cell Signal. 77:109819.; ; Li HS et al, (2019) J Neurosci. 39:5773-5793.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_002731.4
Uniprot Accession Number
P41743
Species
Human
N Terminal Sequence
Met 1
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