Recombinant human ALT2/GPT2 protein

Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, GPT 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2, AAT2, ALT2

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 100unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

60.3 kDa (546aa)

Sohocki M.M. et al. (1997) Genomics 40: 247-252.; ; Matthews C.C. et al. (2003) Hepatology. 39: 1297-1302.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 30% glycerol, 2mM DTT, 0.2M NaCl

Metabolism

NP_597700

Q8TD30

Human