Recombinant human ALT2/GPT2 protein
Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney. Recombinant human GPT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C
Product Name Alternative
Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, GPT 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2, AAT2, ALT2
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
0.5 mg/mL (determined by Bradford assay)
Purity
> 90% by SDS-PAGE
Bioactivity
Specific activity is > 100unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C
Molecular Weight
60.3 kDa (546aa)
Additionnal Information
References & Citations
Sohocki M.M. et al. (1997) Genomics 40: 247-252.; ; Matthews C.C. et al. (2003) Hepatology. 39: 1297-1302.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 30% glycerol, 2mM DTT, 0.2M NaCl
Scientific Category
Metabolism
NCBI Accession Number
NP_597700
Uniprot Accession Number
Q8TD30
Species
Human
AA Sequence
Frequently Asked Questions
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