Recombinant human ALT2/GPT2 protein

Alanine aminotransferase 2, also known as GPT2, catalyzes the reversible transamination between alanine and 2-oxoglutarate to form pyruvate and glutamate. Subsequently, they play a key role in the intermediary metabolism of glucose and amino acids. GPT2 expression is high in muscle, fat and kidney. Recombinant human GPT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 100units/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

Product Name Alternative

Alanine aminotransferase 2, Glutamate pyruvate transaminase 2, GPT 2, Glutamic--alanine transaminase 2, Glutamic--pyruvic transaminase 2, AAT2, ALT2

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.5 mg/mL (determined by Bradford assay)

Purity

> 90% by SDS-PAGE

Bioactivity

Specific activity is > 100unit/mg, and is defined as the amount of enzyme that cleaves 1umole of L-Alanine to L-Glutamate per minute at pH 7.5 at 37C

Molecular Weight

60.3 kDa (546aa)

Additionnal Information

AAT 2, AAT2, Alanine aminotransferase 2, ALT 2, ALT2, Glutamate pyruvate transaminase 2, Glutamic alanine transaminase 2, Glutamic-alanine transaminase 2, Glutamic--alanine transaminase 2, Glutamic-pyruvic transaminase 2, Glutamic--pyruvic transaminase 2, GPT 2, GPT2, Glutamic pyruvic transaminase 2, ATGP4079-10 µg, ATGP4079-20 µg, ATGP4079-50 µg, ATGP4079-100 µg, ATGP4079-250 µg, ATGP4079-500 µg, ATGP4079-1 mg, ATGP4079-010, ATGP4079-020, ATGP4079-050, ATGP4079-100, ATGP4079-250, ATGP4079-500, ATGP4079-01M

References & Citations

Sohocki M.M. et al. (1997) Genomics 40: 247-252.; ; Matthews C.C. et al. (2003) Hepatology. 39: 1297-1302.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 30% glycerol, 2mM DTT, 0.2M NaCl

Scientific Category

Metabolism

NCBI Accession Number

NP_597700

Uniprot Accession Number

Q8TD30

Species

Human

AA Sequence

MQRAAAL VRRGCGPRTP SSWGRSQSSA AAEASAVLKV RPERSRRERI LTLESMNPQV KAVEYAVRGP IVLKAGEIEL ELQRGIKKPF TEVIRANIGD AQAMGQQPIT FLRQVMALCT YPNLLDSPSF PEDAKKRARR ILQACGGNSL GSYSASQGVN CIREDVAAYI TRRDGGVPAD PDNIYLTTGA SDGISTILKI LVSGGGKSRT GVMIPIPQYP LYSAVISELD AIQVNYYLDE ENCWALNVNE LRRAVQEAKD HCDPKVLCII NPGNPTGQVQ SRKCIEDVIH FAWEEKLFLL ADEVYQDNVY SPDCRFHSFK KVLYEMGPEY SSNVELASFH STSKGYMGEC GYRGGYMEVI NLHPEIKGQL VKLLSVRLCP PVSGQAAMDI VVNPPVAGEE SFEQFSREKE SVLGNLAKKA KLTEDLFNQV PGIHCNPLQG AMYAFPRIFI PAKAVEAAQA HQMAPDMFYC MKLLEETGIC VVPGSGFGQR EGTYHFRMTI LPPVEKLKTV LQKVKDFHIN FLEKYA