Recombinant bovine TNF-alpha protein

TNF-alpha, also known as tumor necrosis factor, is the prototypic ligand of the TNF superfamily. It is a pleiotropic molecule that plays a central role in inflammation, apoptosis, and immune system development. This protein is produced by a wide variety of immune and epithelial cell types. It is assembled intracellularly to form a noncovalently linked homotrimer which is expressed on the cell surface. Cell surface TNF can induce the lysis of neighboring tumor cells and virus infected cells, and it can generate its own downstream cell signaling following ligation by soluble TNFR I. It also promotes inflammatory responses by inducing the activation of vascular endothelial cells and macrophages. Recombinant Bovine TNF-alpha was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Bovine TNF-alpha induces cell cytotoxicity in the L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D.

Product Name Alternative

Tumor necrosis factor, Cachectin, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNFSF2, TNF-a, TNFA, TNF

Expression System

E.coli

Antigen Species

Bovine

Tag

Non-Tagged

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured in a cytotoxicity assay using L-929 mouse fibroblast cells in the presence of the metabolic inhibitor actinomycin D. The ED50 range ≤ 15 ng/mL.

Molecular Weight

17.5 kDa (158aa) confirmed by MALDI-TOF

Additionnal Information

TNF-a, TNF-alphaTumor necrosis factor, TNF-a, TNF, TNFa, ATGP4076-10 µg, ATGP4076-20 µg, ATGP4076-50 µg, ATGP4076-100 µg, ATGP4076-250 µg, ATGP4076-500 µg, ATGP4076-1 mg, ATGP4076-10, ATGP4076-20, ATGP4076-50, ATGP4076-100, ATGP4076-250, ATGP4076-500, ATGP4076-1, ATGP3857

References & Citations

Bojarojc-Nosowicz B., et al (2015) . Pol J Vet Sci. 18 (3) : 533-9.; ; Lendez PA., et al (2015) . Arch Virol. 160 (8) : 2001-7.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Hormones & Cytokines

NCBI Accession Number

NP_776391

Uniprot Accession Number

Q06599

Species

Bovine

AA Sequence

MLRSSSQASS NKPVAHVVAD INSPGQLRWW DSYANALMAN GVKLEDNQLV VPADGLYLIY SQVLFRGQGC PSTPLFLTHT ISRIAVSYQT KVNILSAIKS PCHRETPEWA EAKPWYEPIY QGGVFQLEKG DRLSAEINLP DYLDYAESGQ VYFGIIAL