Recombinant human CENPM protein

CENPM, also known as Centromere protein M is involved in centromere assembly and immune response. This protein is an inner protein of the kinetochore, the multi-protein complex that binds spindle microtubules to regulate chromosome segregation during cell division. It was originally identified as a gene highly expressed in b-catenin transformed mouse mammary tissue. CENPM protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Centromere protein M isoform a, bK250D10.2, C22orf18, CENP-M, PANE1

Expression System

E.coli

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Bradford assay)

Purity

> 85% by SDS-PAGE

Molecular Weight

22.1 kDa (203aa) confirmed by MALDI-TOF

Additionnal Information

CENPM, Centromere protein M isoform a, bK250D10.2, C22orf18, CENP-M, PANE1, ATGP2243-10 µg, ATGP2243-20 µg, ATGP2243-50 µg, ATGP2243-100 µg, ATGP2243-250 µg, ATGP2243-500 µg, ATGP2243-1 mg, ATGP2243-10, ATGP2243-20, ATGP2243-50, ATGP2243-100, ATGP2243-250, ATGP2243-500, ATGP2243

References & Citations

Brickner AG, Evans AM, et al. (2006) . Blood. 107 (9) :3779-86. ; ; Bierie B, Edwin M, et al. (2004) . Gene Expr Patterns. 4 (4) :389-95.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Scientific Category

Epigenomics (Transcription & Translation)

NCBI Accession Number

NP_076958

Uniprot Accession Number

Q9NSP4

Species

Human

AA Sequence

MSVLRPL DKLPGLNTAT ILLVGTEDAL LQQLADSMLK EDCASELKVH LAKSLPLPSS VNRPRIDLIV FVVNLHSKYS LQNTEESLRH VDASFFLGKV CFLATGAGRE SHCSIHRHTV VKLAHTYQSP LLYCDLEVEG FRATMAQRLV RVLQICAGHV PGVSALNLLS LLRSSEGPSL EDL