Recombinant human SIRP alpha/CD172a protein

SIRP alpha is a member of the signal-regulatory-protein (SIRP) family, and also belongs to the immunoglobulin superfamily. SIRP family members known to be involved in the negative regulation of receptor tyrosine kinase-coupled signaling processes. SIRP alpha interacts with a broadly expressed transmembrane protein CD47. This interaction negatively controls effector function of innate immune cells such as host cell phagocytosis. This protein can be phosphorylated by tyrosine kinases. The phospho-tyrosine residues of this PTP have been shown to recruit SH2 domain containing tyrosine phosphatases (PTP), and serve as substrates of PTPs. It was found to participate in signal transduction mediated by various growth factor receptors. Recombinant human SIRP alpha, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human SIRP alpha/CD172a is coated at 2 µg/mL (100 ul/well) can bind Human CD47 (CAT# ATGP4070) in a Functional ELISA assay.

Product Name Alternative

SIRPA, Tyrosine-protein phosphatase non-receptor type substrate 1 isoform 1, SHP substrate 1, SHPS-1, Brain Ig-like molecule with tyrosine-based activation motifs, Bit, CD172 antigen-like family member A, Inhibitory receptor SHPS-1, Macrophage fusion receptor, MyD-1 antigen, Signal-regulatory protein alpha-1, Sirp-alpha-1, Signal-regulatory protein alpha-2, Sirp-alpha-2, Signal-regulatory protein alpha-3, Sirp-alpha-3, p84, CD172a, BIT, MFR, MYD1, PTPNS1, SHPS1, SIRP

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Purity

> 95% by SDS - PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human CD47 (CAT# ATGP4070) .

Molecular Weight

39kDa (356aa)

Additionnal Information

ATGP4069-10 µg, ATGP4069-20 µg, ATGP4069-50 µg, ATGP4069-100 µg, ATGP4069-250 µg, ATGP4069-500 µg, ATGP4069-1 mg, ATGP4069-10, ATGP4069-20, ATGP4069-50, ATGP4069-100, ATGP4069-250, ATGP4069-500, ATGP4069-1

References & Citations

Timms J.F., Swanson K.D., et al. (1999) Curr. Biol. 9:927-930.; ; Barclay, A.N. & M.H. Brown (2006) Nat. Rev. Immunol. 6:457.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Adhesion & Movement, Neuroscience

NCBI Accession Number

NP_542970.1

Uniprot Accession Number

P78324

Species

Human

N Terminal Sequence

Asp

AA Sequence

GVAGEEE LQVIQPDKSV LVAAGETATL RCTATSLIPV GPIQWFRGAG PGRELIYNQK EGHFPRVTTV SDLTKRNNMD FSIRIGNITP ADAGTYYCVK FRKGSPDDVE FKSGAGTELS VRAKPSAPVV SGPAARATPQ HTVSFTCESH GFSPRDITLK WFKNGNELSD FQTNVDPVGE SVSYSIHSTA KVVLTREDVH SQVICEVAHV TLQGDPLRGT ANLSETIRVP PTLEVTQQPV RAENQVNVTC QVRKFYPQRL QLTWLENGNV SRTETASTVT ENKDGTYNWM SWLLVNVSAH RDDVKLTCQV EHDGQPAVSK SHDLKVSAHP KEQGSNTAAE NTGSNERNIY