Recombinant canine IL-17A protein

IL-17A, also known as CTLA8, is a member of the IL-17 family of cytokines. This family demonstrate a structural motif termed a cysteine knot that also characterizes a large superfamily of growth factors. The IL-17 family of cytokines includes six members, IL-17A, IL-17B, IL-17C, IL-17D, IL-17E/IL-25, and IL-17F. IL-17A main funcion is stimulates production of IL-6, prostaglandin E and nitric oxide. And other inflammatory cytokines (TNF-alpha, IL-1 beta and IFN -gamma) leads to up-regulation of gene expression and progression and amplification of local inflammation. In addition, it serves induction of co-stimulatory molecules such as ICAM-1 and other cytokines, thereby supporting T cell activation. IL-17 expression has been associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis. Recombinant canine IL-17A, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Canine IL-17A stimulates IL-6 secretion of the NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 10 ng/mL.

Product Name Alternative

IL17A, IL17, IL-17A, Interleukin 17A, Interleukin-17A, cytotoxic T-lymphocyte-associated serine esterase 8, CTLA-8, CTLA8

Expression System

HEK293

Antigen Species

Canine

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

The activity is determined by the IL-6 ELISA in a using NIH/3T3 mouse embryonic fibroblast cells. The ED50 range ≤ 10 ng/mL.

Molecular Weight

15.6kDa (133aa)

Additionnal Information

ATGP4068-10 µg, ATGP4068-20 µg, ATGP4068-50 µg, ATGP4068-100 µg, ATGP4068-250 µg, ATGP4068-500 µg, ATGP4068-1 mg, ATGP4068-10, ATGP4068-20, ATGP4068-50, ATGP4068-100, ATGP4068-250, ATGP4068-500, ATGP4068-1

References & Citations

Moseley TA., et al, (2003) Cytokine & Growth Factor Reviews. 14: 155–174.; ; Afzali, B. et al. (2007) Clin. Exp. Immunol. 148:32-46.; ; Fossiez, F. et al. (1996) J. Exp. Med. 183:2593-2603.;