Recombinant human CCL2/MCP-1 protein
CCL2 (Chemokine ligand 2), as known as MCP-1 (monocyte chemotactic protein-1), is a small cytokine belonging to the CC chemokine family. This protein recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. It binds to chemokine receptors CCR2 and CCR4. It also induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily due to interaction with CCR2 receptors on these cells. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. Recombinant human CCL2/JE/MCP-1, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CCL2/MCP-1 chemoattracts the the THP-1 human acute monocytic leukemia cells. The ED50 range ≤ 30 ng/mL.
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS - PAGE
Bioactivity
Measured its ability to chemoattract using THP-1 human acute monocytic leukemia cells. The ED50 range ≤ 30 ng/mL.
Molecular Weight
9.5kDa (82aa)
Additionnal Information
References & Citations
Foresti ML, Arisi GM, et al. (2009) . J Neuroinflammation. 6:40.; ; Semple BD, Bye N, et al. (2010) . J Cereb Blood Flow Metab. 30:769-782.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology, Hormones & Cytokines
NCBI Accession Number
NP_002973.1
Uniprot Accession Number
P13500
Species
Human
N Terminal Sequence
Gln 24
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