Recombinant human CCL2/MCP-1 protein

CCL2 (Chemokine ligand 2), as known as MCP-1 (monocyte chemotactic protein-1), is a small cytokine belonging to the CC chemokine family. This protein recruits monocytes, memory T cells, and dendritic cells to sites of tissue injury and infection. It binds to chemokine receptors CCR2 and CCR4. It also induces enzyme and cytokine release by monocytes, NK cells and lymphocytes, and histamine release by basophils, primarily due to interaction with CCR2 receptors on these cells. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. Recombinant human CCL2/JE/MCP-1, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CCL2/MCP-1 chemoattracts the the THP-1 human acute monocytic leukemia cells. The ED50 range ≤ 30 ng/mL.

Product Name Alternative

SMC-CF, Small inducible cytokine subfamily A member 2, Small inducible cytokine A2, SCYA2, Monocyte secretory protein JE, Monocyte chemotactic protein 1 homologous to mouse Sig-je, Monocyte chemotactic and activating factor, MGC9434, MCP-1, MCP1, MCAF, HC11, GDCF-2, C-C motif chemokine ligand 2

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Measured its ability to chemoattract using THP-1 human acute monocytic leukemia cells. The ED50 range ≤ 30 ng/mL.

Molecular Weight

9.5kDa (82aa)

Additionnal Information

ATGP4067-10 µg, ATGP4067-20 µg, ATGP4067-50 µg, ATGP4067-100 µg, ATGP4067-250 µg, ATGP4067-500 µg, ATGP4067-1 mg, ATGP4067-10, ATGP4067-20, ATGP4067-50, ATGP4067-100, ATGP4067-250, ATGP4067-500, ATGP4067-1