What is the catalog number for Recombinant human NKp80/KLRF1 protein?

The catalog number for Recombinant human NKp80/KLRF1 protein is ATGP4063-020.

What sizes are available for Recombinant human NKp80/KLRF1 protein?

Recombinant human NKp80/KLRF1 protein is available in the following size(s): 20 µg.

Is Recombinant human NKp80/KLRF1 protein in stock?

Recombinant human NKp80/KLRF1 protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human NKp80/KLRF1 protein

NKp80/KLRF1, also known as killer cell lectin-like receptor subfamily F member 1, is a member of the C-type lectin family. Multiple splicing variants produce four isoforms for NKp80/KLRF1 and isoform 1 is the standard protein. It is expressed on NK cells and a subset of T lymphocytes whereas it is not expressed on B lymphocytes, monocytes and granulocytes. It stimulates NK cell cytotoxicity and induces calcium influx after triggering by appropriate antibodies. It works through interaction with its ligand, AICL (activation-induced C-type lectin), which is selectively expressed on myeloid cells. NKp80-AICL interaction regulates the immune responses at sites of inflammation by stimulating the release of proinflammatory cytokines. Recombinant human NKp80/KLRF1, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human KLRF1 stimulates cell adhesion of the U937 human histiocytic lymphoma cells. This effect is more to 60%.

Product Name Alternative

Killer cell lectin-like receptor F1, Killer cell lectin-like receptor subfamily F member 1, Killer cell lectin-like receptor subfamily F member 1 isoform 1, Lectin-like receptor F1, Activating coreceptor NKp80, NKp80, KLRF1, C-type lectin domain family 5 member C, CLEC5C, ML, CLEC5CMGC119908, MGC119907, MGC119909

Expression System

HEK293

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 85% by SDS - PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to human KLRF1 coated plates 2 µg/mL. This effect is more to 60%.

Molecular Weight

47.1kDa (414aa)

Additionnal Information

ATGP4063-10 µg, ATGP4063-20 µg, ATGP4063-50 µg, ATGP4063-100 µg, ATGP4063-250 µg, ATGP4063-500 µg, ATGP4063-1 mg, ATGP4063-10, ATGP4063-20, ATGP4063-50, ATGP4063-100, ATGP4063-250, ATGP4063-500, ATGP4063-1

References & Citations

Welte S. et al. (2006) Nat. Immunol. 7:1334-1342.; ; Roda-Navarro P. et al. (2000) Eur. J. Immunol. 30:568–576.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_057607.1

Uniprot Accession Number

Q9NZS2

Species

Human

N Terminal Sequence

Asp

AA Sequence

LLVSQGV LLKCQKGSCS NATQYEDTGD LKVNNGTRRN ISNKDLCASR SADQTVLCQS EWLKYQGKCY WFSNEMKSWS DSYVYCLERK SHLLIIHDQL EMAFIQKNLR QLNYVWIGLN FTSLKMTWTW VDGSPIDSKI FFIKGPAKEN SCAAIKESKI FSETCSSVFK WICQY