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Recombinant human Akt3 protein

Akt3, also known as RAC-gamma serine/threonine-protein kinase, is a member of the Akt subfamily of serine/threonine protein kinases. It is one of 3 closely related serine/threonine-protein kinases (Akt1, Akt2 and Akt3) called the Akt kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Also this is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Akt3 is the least studied Akt isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. Recombinant human Akt3, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

RAC-gamma serine/threonine-protein kinase, Protein kinase Akt-3, Protein kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, RAC-gamma serine/threonine-protein kinase isoform 1, v-akt murine thymoma viral oncogene homolog 3, MPPH, MPPH2, PKB-GAMMA, PKBG, PRKBG, RAC-gamma, AKT serine/threonine kinase 3

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Molecular Weight

56.5kDa (485aa)

Additionnal Information

ATGP4061-10 µg, ATGP4061-20 µg, ATGP4061-50 µg, ATGP4061-100 µg, ATGP4061-250 µg, ATGP4061-500 µg, ATGP4061-1 mg, ATGP4061-10, ATGP4061-20, ATGP4061-50, ATGP4061-100, ATGP4061-250, ATGP4061-500, ATGP4061-1

References & Citations

Nakatani K., et al, (1999) J. Biol. Chem. 274: 21528–21532.; ; Brodbeck D., et al, (1999) J.Biol. Chem. 274: 9133–9136.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction

NCBI Accession Number

NP_005456.1

Uniprot Accession Number

Q9Y243

Species

Human

N Terminal Sequence

Met 1

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