Recombinant human Akt3 protein
Akt3, also known as RAC-gamma serine/threonine-protein kinase, is a member of the Akt subfamily of serine/threonine protein kinases. It is one of 3 closely related serine/threonine-protein kinases (Akt1, Akt2 and Akt3) called the Akt kinase, and which regulate many processes including metabolism, proliferation, cell survival, growth and angiogenesis. Also this is mediated through serine and/or threonine phosphorylation of a range of downstream substrates. Akt3 is the least studied Akt isoform. It plays an important role in brain development and is crucial for the viability of malignant glioma cells. Recombinant human Akt3, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS - PAGE
Molecular Weight
56.5kDa (485aa)
Additionnal Information
References & Citations
Nakatani K., et al, (1999) J. Biol. Chem. 274: 21528–21532.; ; Brodbeck D., et al, (1999) J.Biol. Chem. 274: 9133–9136.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Signal Transduction
NCBI Accession Number
NP_005456.1
Uniprot Accession Number
Q9Y243
Species
Human
N Terminal Sequence
Met 1
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