Recombinant human TNFRSF7/CD27 protein
CD27, as known as TNFRSF7, is a member of the tumor necrosis factor receptor superfamily. It has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. Also, this protein plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD 70 on lymphocytes and dendritic cells. It binds to ligand CD70, and CD27/CD70 co-stimulatory system promote T, B and NK cell responses and particularly the CTL response, and is a target in cancer immunotherapy. Recombinant human CD27, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CD27 Ligand/TNFSF7 (CAT# ATGP3336) is coated at 10 µg/mL (100 ul/well) can bind Human CD27/TNFRSF7. The ED50 range ≤ 0.8 µg/mL.
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS - PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human CD27 Ligand/TNFSF7 (CAT# ATGP3336) . The ED50 range ≤ 0.8 µg/mL.
Molecular Weight
46.2kDa (411aa)
Additionnal Information
References & Citations
Clemente A., et al. (2013) Clin. Exp. Immunol. 174:97-108.; ; Huang J., et al. (2013) J. Immunol. 190:6250-6258.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_001233.1
Uniprot Accession Number
P26842
Species
Human
N Terminal Sequence
Ala 20
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