Recombinant human TNFRSF7/CD27 protein

CD27, as known as TNFRSF7, is a member of the tumor necrosis factor receptor superfamily. It has been shown to play roles in lymphoid proliferation, differentiation, and apoptosis.It has an important role in the generation of T cell immunity and is an robust marker for normal memory B cells. Also, this protein plays a key role in regulating B-cell differentiation, activation and immunoglobulin synthesis. As a T and B cell co-stimulatory molecule, the activity of CD27 is governed by its TNF-like ligand CD 70 on lymphocytes and dendritic cells. It binds to ligand CD70, and CD27/CD70 co-stimulatory system promote T, B and NK cell responses and particularly the CTL response, and is a target in cancer immunotherapy. Recombinant human CD27, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human CD27 Ligand/TNFSF7 (CAT# ATGP3336) is coated at 10 µg/mL (100 ul/well) can bind Human CD27/TNFRSF7. The ED50 range ≤ 0.8 µg/mL.

Product Name Alternative

CD27L receptor, T-cell activation antigen CD27, T14, Tumor necrosis factor receptor superfamily member 7, TNFRSF7, T cell antivation antigen S152, TP55

Expression System

HEK293

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS - PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human CD27 Ligand/TNFSF7 (CAT# ATGP3336) . The ED50 range ≤ 0.8 µg/mL.

Molecular Weight

46.2kDa (411aa)

Additionnal Information

ATGP4060-10 µg, ATGP4060-20 µg, ATGP4060-50 µg, ATGP4060-100 µg, ATGP4060-250 µg, ATGP4060-500 µg, ATGP4060-1 mg, ATGP4060-10, ATGP4060-20, ATGP4060-50, ATGP4060-100, ATGP4060-250, ATGP4060-500, ATGP4060-1