What is the catalog number for Recombinant human ENPP-2/Autotaxin protein?

The catalog number for Recombinant human ENPP-2/Autotaxin protein is ATGP4057-020.

What sizes are available for Recombinant human ENPP-2/Autotaxin protein?

Recombinant human ENPP-2/Autotaxin protein is available in the following size(s): 20 µg.

Is Recombinant human ENPP-2/Autotaxin protein in stock?

Recombinant human ENPP-2/Autotaxin protein is currently made to order / not held in immediate stock. Contact us for lead time.

Recombinant human ENPP-2/Autotaxin protein

ENPP-2, also known as Autotaxin, belongs to the ectonucleotide pyrophosphatase/phosphodiesterase (NPP) family. ENPP-2 is able to cleave the phosphodiester bond between the α and the β position of triphosphate nucleotides, acting as an ectonucleotide phosphodiesterase producing pyrophosphate , as most members of the ENPP family. It is unlike ENPP-1 and ENPP-3, has weak activity against nucleotides, but exhibits a lysophospholipase D activity which allows the formation of lysophosphatidic acid (LPA) and choline from lysophosphatidylcholine. Also, ENPP-2 and LPA are involved in numerous inflammatory-driven diseases such as asthma and arthritis. Recombinant human ENPP-2/Autotaxin, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 15,000 units/mg, and defined as the amount of enzyme that hydrolyze 1nmole of bis (p-Nitrophenyl) phosphate per minute at pH8.7 at 37°C.

Product Name Alternative

Ectonucleotide pyrophosphatase/phosphodiesterase family member 2 isoform 2, ectonucleotide pyrophosphatase/phosphodiesterase 2, ENPP2, E-NPP 2, AUTOTAXIN, Extracellular lysophospholipase D, LysoPLD, ENPP2, ATX, PDNP2, ATX-X, NPP2, PD-IALPHA

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Specific activity is > 15,000 units/mg, and defined as the amount of enzyme that hydrolyze 1nmole of bis (p-Nitrophenyl) phosphate per minute at pH8.7 at 37°C.

Molecular Weight

94.9kDa (825aa)

Additionnal Information

ATGP4057-10 µg, ATGP4057-20 µg, ATGP4057-50 µg, ATGP4057-100 µg, ATGP4057-250 µg, ATGP4057-500 µg, ATGP4057-1 mg, ATGP4057-10, ATGP4057-20, ATGP4057-50, ATGP4057-100, ATGP4057-250, ATGP4057-500, ATGP4057-1

References & Citations

Benesch MG., et al, (2014) FEBS Letters. 588:2712-2727.; ; Kawagoe H., et al, (1995) Genomics. 30:380-384.; ; Benesch MG., et al, (2015) Journal of Lipid Research. 56:1134-1144.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Cardiovascular

NCBI Accession Number

NP_001035181.1

Uniprot Accession Number

Q13822

Species

Human

N Terminal Sequence

Asp

AA Sequence

MDSPWTN ISGSCKGRCF ELQEAGPPDC RCDNLCKSYT SCCHDFDELC LKTARGWECT KDRCGEVRNE ENACHCSEDC LARGDCCTNY QVVCKGESHW VDDDCEEIKA AECPAGFVRP PLIIFSVDGF RASYMKKGSK VMPNIEKLRS CGTHSPYMRP VYPTKTFPNL YTLATGLYPE SHGIVGNSMY DPVFDATFHL RGREKFNHRW WGGQPLWITA TKQGVKAGTF FWSVVIPHER RILTILQWLT LPDHERPSVY AFYSEQPDFS GHKYGPFGPE MTNPLREIDK IVGQLMDGLK QLKLHRCVNV IFVGDHGMED VTCDRTEFLS NYLTNVDDIT LVPGTLGRIR SKFSNNAKYD PKAIIANLTC KKPDQHFKPY LKQHLPKRLH YANNRRIEDI HLLVERRWHV ARKPLDVYKK PSGKCFFQGD HGFDNKVNSM QTVFVGYGST FKYKTKVPPF ENIELYNVMC DLLGLKPAPN NGTHGSLNHL LRTNTFRPTM PEEVTRPNYP GIMYLQSDFD LGCTCDDKVE PKNKLDELNK RLHTKGSTEE RHLLYGRPAV LYRTRYDILY HTDFESGYSE IFLMPLWTSY TVSKQAEVSS VPDHLTSCVR PDVRVSPSFS QNCLAYKNDK QMSYGFLFPP YLSSSPEAKY DAFLVTNMVP MYPAFKRVWN YFQRVLVKKY ASERNGVNVI SGPIFDYDYD GLHDTEDKIK QYVEGSSIPV PTHYYSIITS CLDFTQPADK CDGPLSVSSF ILPHRPDNEE SCNSSEDESK WVEELMKMHT ARVRDIEHLT SLDFFRKTSR SYPEILTLKT YLHTYESEI