Recombinant mouse ALCAM/CD166 protein

ALCAM (Activated leukocyte cell adhesion molecule), also known as CD166 antigen, is a member of the Ig CAM family within the immunoglobulin superfamily. It interacts with CD6 that promotes T-cell activation and proliferation and plays a role in the binding of T- and B-cells to activated leukocytes. While expressed in a wide variety of tissues, ALCAM is expressed on activated T cells, activated monocytes, epithelial cells, fibroblasts, neurons, melanoma cells, and also in sweat and sebaceous glands. Recently, CD166 is regarded as a potential novel breast cancer indicator and therapeutic target. Recombinant mouse ALCAM, fused to hIgG-His-tag at C-terminus, was expressed in Insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse ALCAM stimulates inhibit cell adhesion of the Jurkat human acute T cell leukemia cells by CD6. This effect is more to 50%.

Product Name Alternative

Activated leukocyte cell adhesion molecule, ALCAM, Alcam, CD166 antigen, CD166 antigen isoform1, CD166, BEN, Protein DM-GRASP, AI853494 Protein, MuSC, SC1

Expression System

Baculovirus

Antigen Species

Mouse

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Bradford assay)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Measured by its ability to block the adhesion of Jurkat human acute T cell leukemia cells to immobilized Recombinant Human CD6 (CAT# ATGP4005) . When cells are added to 1 µg/mL of mouse ALCAM binded to CD6, This effect is more to 50%.

Molecular Weight

83.1kDa (739aa)

Additionnal Information

ATGP4055-10 µg, ATGP4055-20 µg, ATGP4055-50 µg, ATGP4055-100 µg, ATGP4055-250 µg, ATGP4055-500 µg, ATGP4055-1 mg, ATGP4055-10, ATGP4055-20, ATGP4055-50, ATGP4055-100, ATGP4055-250, ATGP4055-500, ATGP4055-1

References & Citations

Bowen, M.A. et al. (1995) J. Exp. Med. 181:2213-2220.; ; Castro, M.A.A. et al. (2007) J. Immunol. 178:4351-4361.; ; Degen, W.G. et al. (1998) Am. J. Pathol. 152:805-813.; ; King, J.A. et al. (2010) Mol. Cancer 9:266.;