Recombinant mouse Osteopontin/OPN protein
Osteopontin, also known as SPP1, is major non-collagenous bone protein that binds tightly to hydroxyapatite. This protein appears to form an integral part of the mineralized matrix. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration. Osteopontin acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. Also it is essential in the pathway that leads to type 1 immunity. It is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Recombinant mouse Osteopontin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse SPP1 stimulates cell adhesion of the HEK293 human embryonic kidney cells. The ED50 range ≤ 1.5 µg/mL.
Product Name Alternative
Expression System
HEK293
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
>90% by SDS-PAGE
Bioactivity
Molecular Weight
31.8kDa (287aa)
Additionnal Information
References & Citations
Icer MA, et al, (2018) Clin Biochem. 51:17-24.; ; Kaleta B. (2019) Inflamm Res. 68:93-102.; ; Lamort AS, et al, (2019) Cells. 8:815.;
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Angiogenesis, Cardiovascular, Adhesion & Movement
NCBI Accession Number
NP_033289.2
Uniprot Accession Number
P10923
Species
Mouse
N Terminal Sequence
Asp
AA Sequence
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