Recombinant mouse Osteopontin/OPN protein

Osteopontin, also known as SPP1, is major non-collagenous bone protein that binds tightly to hydroxyapatite. This protein appears to form an integral part of the mineralized matrix. It inhibits bone mineralization and kidney stone formation, and promotes inflammation and cell adhesion and migration. Osteopontin acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10. Also it is essential in the pathway that leads to type 1 immunity. It is expressed and secreted by various cells, and has a role in cell adhesion, chemotaxis, prevention of apoptosis, invasion, migration and anchorage-independent growth of tumor cells. Recombinant mouse Osteopontin, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse SPP1 stimulates cell adhesion of the HEK293 human embryonic kidney cells. The ED50 range ≤ 1.5 µg/mL.

Product Name Alternative

Secreted phosphoprotein 1, 2AR, Ap1-1, BNSP, Bsp, BSP1, Eta, ETA-1, mi, OP, Opn, Opn1, Spp-1, osteopontine isoform 4, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, Osteoponitin, Spp1

Expression System

HEK293

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

>90% by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of HEK293 human embryonic kidney cells. When cells are added to mouse SPP1 coated plates. The ED50 range ≤ 1.5 µg/mL.

Molecular Weight

31.8kDa (287aa)

Additionnal Information

Secreted phosphoprotein 1, 2AR, Ap, Ap1-1, B , BNSP, Bsp, BSP1, Eta, ETA-, ETA-1, mi, OP, Opn, Opn1, ost, R, Ric, Sp, Spp-1, osteopontine isoform 4 precursor, Bone sialoprotein 1, Calcium oxalate crystal growth inhibitor protein, Early T-lymphocyte activation 1 protein, Minopontin, ATGP4052-10 µg, ATGP4052-20 µg, ATGP4052-50 µg, ATGP4052-100 µg, ATGP4052-250 µg, ATGP4052-500 µg, ATGP4052-1 mg, ATGP4052-10, ATGP4052-20, ATGP4052-50, ATGP4052-100, ATGP4052-250, ATGP4052-500, ATGP4052-1

References & Citations

Icer MA, et al, (2018) Clin Biochem. 51:17-24.; ; Kaleta B. (2019) Inflamm Res. 68:93-102.; ; Lamort AS, et al, (2019) Cells. 8:815.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Angiogenesis, Cardiovascular, Adhesion & Movement

NCBI Accession Number

NP_033289.2

Uniprot Accession Number

P10923

Species

Mouse

N Terminal Sequence

Asp

AA Sequence

LPVKVTD SGSSEEKLYS LHPDPIATWL VPDPSQKQNL LAPQNAVSSE EKDDFKQETL PSNSNESHDH MDDDDDDDDD DGDHAESEDS VDSDESDESH HSDESDETVT ASTQADTFTP IVPTVDVPNG RGDSLAYGLR SKSRSFQVSD EQYPDATDED LTSHMKSGES KESLDVIPVA QLLSMPSDQD NNGKGSHESS QLDEPSLETH RLEHSKESQE SADQSDVIDS QASSKASLEH QSHKFHSHKD KLVLDPKSKE DDRYLKFRIS HELESSSSEV N