Recombinant human Ribonuclease A/RNASE 1 protein
RNASE1, also known as ribonuclease A, is a member of pancreatic ribonuclease enzyme family. It is a relatively small protein and is a basic protein (pI = 9.63) . Also, It has four disulfide bonds in its native state. It cleaves specifically after pyrimidine nucleotides. Cleavage takes place in two steps: first, the 3’,5’-phosphodiester bond is cleaved to generate a 2’,3’-cyclic phosphodiester intermediate; second, the cyclic phosphodiester is hydrolyzed to a 3’-monophosphate. (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG) The highest activity is exhibited with single stranded RNA. It can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. Recombinant human RNASE1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Specific activity is > 3 X 10^6 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25C.
Product Name Alternative
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Enzyme Activity
Concentration
1 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
90% by SDS – PAGE
Bioactivity
Specific activity is > 5 X 10^4 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25C.
Molecular Weight
15.3kDa (134aa)
Additionnal Information
References & Citations
Raines RT (1998) Chem. Rev. 98:1045–1066.; ; JC Kosgey et al, (2020) Int. J. Biol. 160:1042-1049.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Enzymes & Proteases, Epigenomics (Transcription & Translation)
NCBI Accession Number
NP_937878.1
Uniprot Accession Number
P07998
Species
Human
N Terminal Sequence
Lys29
Frequently Asked Questions
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