Recombinant human Ribonuclease A/RNASE 1 protein

RNASE1, also known as ribonuclease A, is a member of pancreatic ribonuclease enzyme family. It is a relatively small protein and is a basic protein (pI = 9.63) . Also, It has four disulfide bonds in its native state. It cleaves specifically after pyrimidine nucleotides. Cleavage takes place in two steps: first, the 3’,5’-phosphodiester bond is cleaved to generate a 2’,3’-cyclic phosphodiester intermediate; second, the cyclic phosphodiester is hydrolyzed to a 3’-monophosphate. (For example pG-pG-pC-pA-pG will be cleaved to give pG-pG-pCp and A-pG) The highest activity is exhibited with single stranded RNA. It can also hydrolyze RNA from protein samples. RNase A can be inhibited by alkylation of His12 and His119 and activated by potassium and sodium salts. Recombinant human RNASE1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Specific activity is > 3 X 10^6 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25C.

Product Name Alternative

RNASE1, ribonuclease A family member 1, pancreatic, RAC1, RIB1, RNS1, ribonuclease pancreatic, HP-RNase, RIB-1, RNase UpI-1, RNase 1, Ribonuclease A, RNase A

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

90% by SDS – PAGE

Bioactivity

Specific activity is > 5 X 10^4 unit/mg, and is defined as the amount of enzyme that cleaves 1.0 pmole of RNase probe per minute at 25C.

Molecular Weight

15.3kDa (134aa)

Additionnal Information

ATGP4051-10 µg, ATGP4051-20 µg, ATGP4051-50 µg, ATGP4051-100 µg, ATGP4051-250 µg, ATGP4051-500 µg, ATGP4051-1 mg, ATGP4051-10, ATGP4051-20, ATGP4051-50, ATGP4051-100, ATGP4051-250, ATGP4051-500, ATGP4051-1