Recombinant human Casein kinase 2 beta/CSNK2B protein

Casein Kinase 2 (CK2, also called PKCK2) is a ubiquitous Ser/Thr kinase expressed in all eukaryotes. CK2 is a tetramer composed of two catalytic kinase domains, alpha subunits, and two identical regulatory beta subunits. It has been implicated in cell cycle control, DNA repair, regulation of the circadian rhythm, and other cellular processes. The beta subunit itself does not have kinase activity, but confers stability to the CK2 alpha subunit and is involved in activity and substrate specificity. Recombinant human CK2beta was expressed in E. coli and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Casein kinase 2 beta polypeptide, Casein kinase II subunit beta, CK II beta, Phosvitin, Protein G5a, Ckb1, Ckb2, CK2N, G5A

Expression System

E.coli

Antigen Species

Human

Tag

Non-Tagged

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Bradford assay)

Purity

> 95% by SDS-PAGE

Molecular Weight

24.9 kDa (215aa) confirmed by MALDI-TOF

Additionnal Information

Casein Kinase2 b, PKCK2 Beta, PKCK2B, CK2N, CSK2B, MGC138222, MGC138224, G5A, Phosvitin, Casein Kinase 2 beta, Casein Kinase 2B, Casein Kinase 2 beta, Casein kinase 2 beta polypeptide, Casein kinase II subunit beta, CK II beta, CK2B, CSNK 2B, CSNK2B, G5A, CKB0801-10 µg, CKB0801-20 µg, CKB0801-50 µg, CKB0801-100 µg, CKB0801-250 µg, CKB0801-500 µg, CKB0801-1 mg, CKB0801-10, CKB0801-20, CKB0801-50, CKB0801-100, CKB0801-250, CKB0801-500, CKB0801-1, ATGP4047-10 µg, ATGP4047-20 µg, ATGP4047-50 µg, ATGP4047-100 µg, ATGP4047-250 µg, ATGP4047-500 µg, ATGP4047-1 mg, ATGP4047-10, ATGP4047-20, ATGP4047-50, ATGP4047-100, ATGP4047-250, ATGP4047-500, ATGP4047-1

References & Citations

Litchfield DW., et al. (2003) . Biochem J. 369 (1) :1-15; ; unger GM., et al. (2004) . Curr. Cancer Drug Targets 4 (1) :77-84;

Other References

Matsumoto M, et al. CSNK2B modulates IRF1 binding to functional DNA elements and promotes basal and agonist-induced antiviral signaling. (Nucleic Acids Res. 2023) {https://pubmed.ncbi.nlm.nih.gov/37094077/}

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 0.2M NaCl, 1mM DTT, 1mM EDTA

Scientific Category

Signal Transduction

NCBI Accession Number

NP_001311

Uniprot Accession Number

P67870

Species

Human

AA Sequence

MSSSEEVSWI SWFCGLRGNE FFCEVDEDYI QDKFNLTGLN EQVPHYRQAL DMILDLEPDE ELEDNPNQSD LIEQAAEMLY GLIHARYILT NRGIAQMLEK YQQGDFGYCP RVYCENQPML PIGLSDIPGE AMVKLYCPKC MDVYTPKSSR HHHTDGAYFG TGFPHMLFMV HPEYRPKRPA NQFVPRLYGF KIHPMAYQLQ LQAASNFKSP VKTIR