Recombinant mouse CTLA4 protein
Ctla-4, also known as cytotoxic T-lymphocyte protein 4 isoform 1, is a type 1 membrane protein and a memberof the immunoglobulin-superfamily. Besides, this protein contains an extra cellular domain, a transmembranedomain and a cytoplasmic tail. It is generally expressed with highest levels in lymphoid tissues. Also, it is similarto T-cell costimulatory protein CD28 since both of the molecules bind to CD80 and CD86 on antigen-presenting cells. CTLA4 and CD28 effectively regulate to T cell responses as opposing signals transmitted through tworelated cell-surface receptors. Recombinant mouse Ctla-4, fused to hIgG-His-tag at C-terminus, was expressed ininsect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse Ctla4 inhibits IL-2 secretion of the stimulated Jurkat human acute T cell leukemia cells with Human B7-1/CD80. The ED50 range ≤150 ng/mL.
Product Name Alternative
Ly-56, Ly-5, Cytotoxic T-lymphocyte-associated antigen 4, Cytotoxic T-lymphocyte protein 4 isoform 1, Ctla-4, CTLA4, CTLA, Cd152
Expression System
Baculovirus
Antigen Species
Mouse
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS - PAGE
Bioactivity
Molecular Weight
40.6kDa (363aa)
Additionnal Information
References & Citations
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_033973.2
Uniprot Accession Number
P09793
Species
Mouse
N Terminal Sequence
Ile 38
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