Recombinant human ErbB4/Her4 protein

ErbB4, also known as Her4, is a receptor tyrosine kinase that is a member of the epidermal growth factor receptor family. ErbB family members serve as receptors for the EGF family of growth factors. It is a single-pass type I transmembrane protein with multiple furin-like cysteine rich domains, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. It is expressed in normal skeletal muscle, heart, pituitary, brain and several breast carcinomas. ErbB4 ligands include the neuregulins, beta-cellulin and heparin-binding EGF-like growth factor (HB-EGF) . Monomeric ErbB4 binds its ligands with low affinity. ErbB4 appears to play important roles in neuronal development, development of the heart and cancer. Single-nucleotide polymorphisms in ERBB4 have also been found in a study of patients with familial amyotrophic lateral sclerosis type 19. Recombinant human ErbB4, fused to hIgG-His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human NRG1/HRG1 (CAT# ATGP3990) is coated at 10 µg/mL (100 ul/well) can bind Human ERBB4. The ED50 range ≤ 0.2 µg/mL.

Product Name Alternative

Receptor tyrosine-protein kinase erbB-4, Proto-oncogene-like protein c-ErbB-4, Tyrosine kinase-type cell surface receptor HER4, p180erbB4, 4ICD, E4ICD, s80HER4, ERBB4, HER4, receptor tyrosine-protein kinase erbB-4 isoform JM-a/CVT-1, receptor tyrosine-protein kinase erbB-4, tyrosine kinase-type cell surface receptor HER4, avian erythroblastic leukemia viral (v-erb-b2) oncogene homolog 4, proto-oncogene-like protein c-ErbB-4, v-erb-a erythroblastic leukemia viral oncogene homolog 4, v-erb-b2 avian erythroblastic leukemia viral oncogene homolog 4, human epidermal growth factor receptor 4, ALS19

Expression System

HEK293

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human NRG1/HRG1 (CAT# ATGP3990) . The ED50 range ≤ 0.2 µg/mL.

Molecular Weight

96.6kDa (863aa)

Additionnal Information

ATGP4044-10 µg, ATGP4044-20 µg, ATGP4044-50 µg, ATGP4044-100 µg, ATGP4044-250 µg, ATGP4044-500 µg, ATGP4044-1 mg, ATGP4044-10, ATGP4044-20, ATGP4044-50, ATGP4044-100, ATGP4044-250, ATGP4044-500, ATGP4044-1

References & Citations

Silberberg G., et al, (2006) American Journal of Medical Genetics Part B. 141B: 142–148.; ; C Rio. et al, (2000) J Biol Chem. 275:10379-10387.; ; Zimonjic DB., et al, (1995) Oncogene. 10:1235–1237.