Recombinant human PGLYRP1/PGRP-S protein

PGLYRP1, also known as peptidoglycan recognition protein 1, is an innate immunity protein that plays several important functions in antimicrobial and antitumor defense systems. It acts as a pattern receptor that binds to murein peptidoglycans of gram-positive bacteria and provides bactericidal activity. PGLYRP1 forms an equimolar complex with HSPA1A and induces programmed cell death through apoptosis and necroptosis in tumor cell lines by activating the TNFR1 receptor on the target cell membrane. This protein also interacts with S100A4 and function as a chmoattractant which induces lymphocyte movement. Recombinant human PGLYRP1, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Peptidoglycan is coated at 10 µg/mL (100 ul/well) can bind Human PGLYRP1. The ED50 range ≤ 30 ng/mL.

Product Name Alternative

PGLYRP, PGRP, PGRP-S, PGRPS, TAG7, TNFSF3L, Peptidoglycan recognition protein 1, peptidoglycan recognition protein short

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Peptidoglycan. The ED50 range ≤ 30 ng/mL.

Molecular Weight

20.5kDa (184aa)

Additionnal Information

ATGP4036-10 µg, ATGP4036-20 µg, ATGP4036-50 µg, ATGP4036-100 µg, ATGP4036-250 µg, ATGP4036-500 µg, ATGP4036-1 mg, ATGP4036-10, ATGP4036-20, ATGP4036-50, ATGP4036-100, ATGP4036-250, ATGP4036-500, ATGP4036-1

References & Citations

Lang MF, et al, (2008) Neurosci Lett. 430:138-141.; ; Sharma P., et al, (2012) Int J Biochem Mol Biol. 3:86-94.; ; Sun Q., et al, (2014) World J Microbiol Biotechnol. 30:263-270.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Apoptosis

NCBI Accession Number

NP_005082.1

Uniprot Accession Number

O75594

Species

Human

N Terminal Sequence

Asp

AA Sequence

QETEDPA CCSPIVPRNE WKALASECAQ HLSLPLRYVV VSHTAGSSCN TPASCQQQAR NVQHYHMKTL GWCDVGYNFL IGEDGLVYEG RGWNFTGAHS GHLWNPMSIG ISFMGNYMDR VPTPQAIRAA QGLLACGVAQ GALRSNYVLK GHRDVQRTLS PGNQLYHLIQ NWPHYRSP