Recombinant mouse Thrombospondin 4/THBS4 protein

Thrombospondin-4, also known as THBS4, is a member of the thrombospondin protein family. This family members are adhesive glycoproteins that mediate cell-to-cell and cell-to-matrix interactions. This protein forms a pentamer and can bind to heparin and calcium. Among them Thrombospondin-4 binds a variety of matrix proteins including Collagens I, II, III, V, Laminin‑1, Fibronectin, and Matrilin‑2. And it plays a role in cardiovascular physiology and neuronal development. It is up‑regulated in the spinal cord following peripheral nerve injury where it contributes to presynaptic hypersensitivity and hyperalgesia. It is also up‑regulated in muscle following denervation. It contributes to the development of inflammation and atherosclerosis by promoting macrophage and neutrophil adhesion to the vasculature. Recombinant mouse Thrombospondin-4, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.