Recombinant human Selenoprotein W/SELENOW protein
SEPW1 is a selenoprotein, which contains a selenocysteine (Sec) residue at its active site. The selenocysteine is encoded by the uGA codon that normally signals translation termination. The 3 uTR of selenoprotein genes have a common stem-loop structure, the sec insertion sequence (SECIS), that is necessary for the recognition of uGA as a Sec codon rather than as a stop signal. This protein shows highest expression in skeletal muscle and heart, and may be involved in oxidation-reduction reactions. A retroprocessed pseudogene, SEPW1P, has been identified and mapped to chromosome 1p35-34. Recombinant human SEPW1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Product Name Alternative
Selenoprotein W, selW, SEPW1, Selenoprotein W1
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE
Concentration
0.25 mg/mL (determined by BRADFORD assay)
Purity
> 95% by SDS-PAGE
Molecular Weight
11.8 kDa (110aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Hawkes, W.C., et al. (2012) J. Biol. Chem. 287 (33), 27371-27379; ; Hawkes, W.C., et al. (2012) J. Cell. Biochem. 113 (1), 61-69
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT
Scientific Category
Other
NCBI Accession Number
NP_003000
Uniprot Accession Number
P63302
Species
Human
N Terminal Sequence
Recombinant Protein
AA Sequence
MALAVRV VYCGACGYKS KYLQLKKKLE DEFPGRLDIC GEGTPQATGF FEVMVAGKLI HSKKKGDGYV DTESKFLKLV AAIKAALAQG
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