Recombinant human L1CAM protein

L1CAM, also known as neural cell adhesion molecule L1 isoform 3, is a cell adhesion receptor of the immunoglobulin superfamily, known for its roles in nerve cell function. It is now recognized to play a key role in cell migration, adhesion, neurite outgrowth, myelination and neuronal differentiation Overexpression of L1CAM in normal and cancer cells increased motility, enhanced growth rate and promoted cell transformation and tumorigenicity. Recombinant human L1CAM, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Neural cell adhesion molecule L1 isoform 3, L1CAM, CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Neuro-2a mouse neuroblastoma cells. When cells are added to human L1CAM coated plates 1 µg/mL. This effect is more to 30%.

Molecular Weight

123.6 kDa (1104aa)

Additionnal Information

ATGP3618, ATGP4032-10 µg, ATGP4032-20 µg, ATGP4032-50 µg, ATGP4032-100 µg, ATGP4032-250 µg, ATGP4032-500 µg, ATGP4032-1 mg, ATGP4032-10, ATGP4032-20, ATGP4032-50, ATGP4032-100, ATGP4032-250, ATGP4032-500, ATGP4032-1