Recombinant porcine IFN-alpha 1/IFNA1 protein

IFN-alpha 1, also known as Interferon alpha-1, belongs to a family of cytokines with potent antiviral , antiproliferative and immunomodulatory properties. IFNs were originally discovered as molecules that could reduce the ability of a normal virus to infect cells, a process called viral interference. IFNs have been classified into two major types of IFNs, type I and type II, based on their interactions to a specific cell surface receptor. The type I IFNs bind to the interferon alpha receptor, which consists of two subunits, IFNAR1 and IFNAR2. The IFN-α proteins are produced mainly by plasmacytoid dendritic cells (pDCs) . They are mainly involved in innate immunity against viral infection. It is also made synthetically as medication in hairy cell leukemia. Recombinant porcine IFN-alpha 1, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Interferon alpha-1, Interferon-alpha, IFN-alpha-1, IFNA1, IFN-ALPHA-1, IFN1@

Expression System

HEK293

Antigen Species

Pocrine

Tag

His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Molecular Weight

20.2kDa (176aa)

Additionnal Information

ATGP4031-10 µg, ATGP4031-20 µg, ATGP4031-50 µg, ATGP4031-100 µg, ATGP4031-250 µg, ATGP4031-500 µg, ATGP4031-1 mg, ATGP4031-10, ATGP4031-20, ATGP4031-50, ATGP4031-100, ATGP4031-250, ATGP4031-500, ATGP4031-1

References & Citations

Lengyel P (1982) Annual Review of Biochemistry. 51:252-282.; ; Pestka S., et al, (1987) Annual Review of Biochemistry. 56:727–777.; ; Isaacs A., et al, (1957) Biological Sciences. 147 (927) :258–267.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology, Hormones & Cytokines

NCBI Accession Number

NP_999558.1

Uniprot Accession Number

Q6VAB8

Species

Pocrine

N Terminal Sequence

Asp

AA Sequence

CDLPQT HSLAHTRALR LLAQMRRISP FSCLDHRRDF GSPHEAFGGN QVQKAQAMAL VHEMLQQTFQ LFSTEGSAAA WNESLLHQFC TGLDQQLRDL EACVMQEAGL EGTPLLEEDS ILAVRKYFHR LTLYLQEKSY SPCAWEIVRA EVMRSFSSSR NLQDRLRKKE