Recombinant human SIRP gamma/CD172g protein
SIRPG, also known as signal-regulatory protein gamma isoform 1, is a member of a closely related family of three cell surface receptors implicated in modulating immune/inflammatory responses. It is the only SIRP known to be expressed on T cells, CD56 bright NK cells and activated NK cells. Expression of SIRPG on T cells suggests a role as an accessory protein interacting with CD47‑expressing antigen presenting cells. Engagement of CD47 by SIRPG was shown to induce apoptosis on T-cell and monocyte cell lines. Recombinant human SIRPG, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CD47 (CAT# ATGP4015) is coated at 1 µg/mL (100 ul/well) can bind Human SIRP gamma/CD172g. The ED range ≤ 1 µg/mL.
Product Name Alternative
Signal-regulatory protein gamma isoform 1, SIRPG, bA77C3.1, CD172g, SIRP-B2, SIRPB2, SIRPgamma
Expression System
Baculovirus
Antigen Species
Human
Tag
HIgG-His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human CD47 (CAT# ATGP4015) . The ED50 range ≤ 1 µg/mL.
Molecular Weight
64 kDa (574aa)
Additionnal Information
References & Citations
Nettleship JE., et al. (2013) BMC Struct Biol. 13:13.; ; Brooke G., et al. (2004) J Immunol. 173:2562-2570.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Scientific Category
Immunology, Adhesion & Movement
NCBI Accession Number
NP_061026.2
Uniprot Accession Number
Q9P1W8
Species
Human
N Terminal Sequence
Ala
AA Sequence
Frequently Asked Questions
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