Recombinant mouse IL-5 Protein

IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. This interleukin produced by type-2 T helper cells and mast cells. It related hematopoietic cytokines that are important for allergic inflammation.. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release. Recombinant mouse IL-5, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Mouse IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 1 ng/mL.

Product Name Alternative

Interleukin-5, B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, TRF, EDF

Expression System

Baculovirus

Antigen Species

Mouse

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 1 ng/mL.

Molecular Weight

13.9kDa (119aa)

Additionnal Information

TRFB cell differentiation factor I, TRF, T-cell replacing factor, T cell replacing factor, Interleukin-5, Interleukin5, Interleukin 5, IL-5, IL5, IL 5, Eosinophil differentiation factor, EDF, Cytotoxic T-lymphocyte inducer, Cytotoxic T lymphocyte inducer, BCGF-II, BCGFII, BCGF II, B-cell growth factor II, B-cell differentiation factor I, B cell growth factor II, B cell differentiation factor I, ATGP4029-10 µg, ATGP4029-20 µg, ATGP4029-50 µg, ATGP4029-100 µg, ATGP4029-250 µg, ATGP4029-500 µg, ATGP4029-1 mg, ATGP4029-010, ATGP4029-020, ATGP4029-050, ATGP4029-100, ATGP4029-250, ATGP4029-500, ATGP4029-01M