Recombinant mouse IL-5 protein
IL5, also known as interleukin-5, is a member of the hematopoietin receptor superfamily and is comprised of a cytokine-specific alpha chain and the common beta chain that is shared among these cytokines for signaling. This interleukin produced by type-2 T helper cells and mast cells. It related hematopoietic cytokines that are important for allergic inflammation.. It is a lineage-specific cytokine for eosinophilpoiesis and plays an important part in diseases associated with increased eosinophils, such as asthma. In humans, IL-5 primarily affects cells of the eosinophilic lineage, and promotes their differentiation, maturation, activation, migration and survival, while in mice IL-5 also enhances Ig class switching and release from B1 cells. IL-5 also promotes differentiation of basophils and primes them for histamine and leukotriene release. Recombinant mouse IL-5, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Mouse IL-5 in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 3 ng/mL.
Product Name Alternative
Interleukin-5, B-cell growth factor II, BCGF-II, Cytotoxic T-lymphocyte inducer, Eosinophil differentiation factor, T-cell replacing factor, TRF, EDF
Expression System
HEK293
Antigen Species
Mouse
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.25 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 90% by SDS - PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 3 ng/mL.
Molecular Weight
14.2kDa (122aa)
Additionnal Information
References & Citations
Martinez-Moczygemba M., et al. (2003) J Allergy Clin Immunol. 112:653-665.; ; Milburn MV., et al. (1993) Nature. 363:172-176.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_034688.1
Uniprot Accession Number
P04401
Species
Mouse
N Terminal Sequence
Asp
AA Sequence
MEIPMST VVKETLTQLS AHRALLTSNE TMRLPVPTHK NHQLCIGEIF QGLDILKNQT VRGGTVEMLF QNLSLIKKYI DRQKEKCGEE RRRTRQFLDY LQEFLGVMST EWAMEG
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