Recombinant human PIGF-2 protein

PIGF, as know as placenta growth factor, is a member of the VEGF sub-family that a key molecule in angiogenesis and vasculogenesis, in particular during embryogenesis. It is ultimately associated with angiogenesis. Specifically, it plays a role in trophoblast growth and differentiation. Also it is a potential biomarker for preeclampsia, a condition in which blood vessels in the placenta are too narrow, resulting in high blood pressure. Under normal physiologic conditions, it is also expressed at a low level in other organs including the heart, lung, thyroid, and skeletal muscle. Recombinant human PIGF, fused to His-tag at C-terminus, was expressed in HEK293 and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

Placental growth factor, placenta growth factor isoform 1, PGF, D12S1900, PGFL, PIGF, PLGF, PlGF-2, SHGC-10760

Expression System

HEK293

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS - PAGE

Molecular Weight

18.3kDa (160aa)

Additionnal Information

ATGP4025-10 µg, ATGP4025-20 µg, ATGP4025-50 µg, ATGP4025-100 µg, ATGP4025-250 µg, ATGP4025-500 µg, ATGP4025-1 mg, ATGP4025-10, ATGP4025-20, ATGP4025-50, ATGP4025-100, ATGP4025-250, ATGP4025-500, ATGP4025-1

References & Citations

Asma Khalil., et al, (2008) PLoS One. 3:e2766.; ; Khurana R., et al, (2005) Circulation. 111:2828-2836.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Angiogenesis, Cardiovascular

NCBI Accession Number

NP_002623.2

Uniprot Accession Number

P49763-3

Species

Human

N Terminal Sequence

Asp

AA Sequence

AVPPQQ WALSAGNGSS EVEVVPFQEV WGRSYCRALE RLVDVVSEYP SEVEHMFSPS CVSLLRCTGC CGDENLHCVP VETANVTMQL LKIRSGDRPS YVELTFSQHV RCECRPLREK MKPERRRPKG RGKRRREKQR PTDCHLCGDA VPRR