Recombinant human ULBP-6/RAET1L Protein
RAET1L, also known as retinoic acid early transcript 1L, linked to the cell membrane by the GPI anchor. This protein functions as a stress-induced ligand for NKG2D receptor. Its expression is, for example, triggered in course of HCMV infection, but HCMV alters its function. HCMV-encoded UL16 glycoprotein retains RAET1L/ULBP6 inside the cells, preventing it from reaching the cell surface and being exposed to cells of the immune system. It had a more restricted expression profile in cell lines and primary human tissues than other NKG2D ligands. RAET1L mRNA shows restricted expression in the trachea and some HPV positive cervical carcinoma and colorectal carcinoma cell lines. Recombinant human RAET1L, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human RAET1L/ULBP6 is coated at 10 µg/mL (100 ul/well) can bind Human NKG2D (CAT# ATGP3662) in a Functional ELISA assay.
Product Name Alternative
Early transcript 1L, ULBP6, UL16-binding protein 6 , RAET1L, retinoic acid early transcript 1L protein, retinoic acid early transcript 1L
Expression System
Baculovirus
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS - PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human NKG2D (CAT# ATGP3662) .
Molecular Weight
22.9kDa (202aa)
Additionnal Information
References & Citations
Eagle R.A., et al. (2009) Eur. J. Immunol. 39:3207.; ; Kondo M., et al. (2010) Immunogenetics 62:441.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Immunology
NCBI Accession Number
NP_570970
Uniprot Accession Number
Q5VY80
Species
Human
N Terminal Sequence
Ala
AA Sequence
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