Recombinant human CD40 Ligand/CD40LG protein
CD40 ligand, also known as CD40LG, is a member of the TNF superfamily and is expressed on activated T cells. CD40LG is expressed as a soluble cytokine as well as a homotrimeric type II transmembrane protein. It has been reported to be important for B cell costimulation following binding of its receptor, CD40. Mutations in the gene encoding for CD154 are implicated in hyper-IgM immunodeficiency syndrome type 1. Recombinant human CD40LG protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human CD40 Ligand/TNFSF5 is coated at 5 µg/mL (100 ul/well) can bind Human CD40/TNFRSF5 (CAT# ATGP4007) in a Functional ELISA assay.
Product Name Alternative
Expression System
E.coli
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Bradford assay)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS-PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human CD40/TNFRSF5 (CAT# ATGP4007) .
Molecular Weight
18.3 kDa (169aa) confirmed by MALDI-TOF
Additionnal Information
References & Citations
Gordon J., et al. (1995) Blood Rev. 9:53-56.; ; Cheng G., et al. (1995) Science. 267:1494-1498.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 40% glycerol, 0.2M NaCl
Scientific Category
Immunology
NCBI Accession Number
NP_000065
Uniprot Accession Number
P29965
Species
Human
AA Sequence
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