Recombinant human IL-17E/IL-25 protein
IL-25, also known as IL-17E, belongs to the IL-17 cytokine family. It support the Th2 immune response, and has been shown to induce the production of IL-4, IL-5 and IL-13. This is in contrast to other IL-17 family members which promote Th1- and Th17-biased inflammation. Because IL-25 promotes the development of a Th2 immune response, it acts to protect against several bowel infections caused by helminths. It is also referred to as the regulator of IL-9 production. IL-25 has been shown to increase the production of IL-9 in Th9 cells. Another function of this protein is the activation of natural lymphoid cells. IL-25 and IL-33 are the most potent activators of ILC2. IL-25 has potent antitumor activity in vivo in several human cancers including melanoma, breast, lung, colon, and pancreatic cancers, suggesting the potential clinical use of IL-17E as an anticancer agent. Recombinant human IL-17E/IL-25, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Human IL-17E/IL-25 is coated at 2 µg/mL (100 ul/well) can bind Human IL-17 RB (CAT# ATGP4039) in a Functional ELISA assay.
Product Name Alternative
Interleukin-25 isoform 1, interleukin 25, IL17E, IL-17E, IL25, IL-25, interleukin-17E, interleukin-25, Macroprhagleukin-25 isoform 2
Expression System
HEK293
Antigen Species
Human
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
0.5 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS - PAGE
Bioactivity
Measured by its binding ability in a functional ELISA with Human IL-17 RB (CAT# ATGP4039)
Molecular Weight
17.8kDa (154aa)
Additionnal Information
References & Citations
Xinyang Song., et al, (2013) Cell Signal. 25:2335-2347.; ; Rickel EA., et al, (2008) J Immunol. 181:4299-4310.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol
Scientific Category
Immunology, Hormones & Cytokines
NCBI Accession Number
NP_073626.1
Uniprot Accession Number
Q9H293
Species
Human
N Terminal Sequence
Asp
AA Sequence
Frequently Asked Questions
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