Recombinant human DR3/TNFRSF25 protein

TNFRSF25, also known as Tumor necrosis factor receptor superfamily member 25, is a member of the TNF receptor superfamily that binds to the TNF-like protein TL1A. TNFRSF25 signals are required to exert T helper cell 2 effector function in Th2-polarized CD4 cells and co-stimulate interleukin-13 production by glycosphingolipid_x0002_activated NKT cells. Its axis is a novel immune pathway that participates in the pathogenesis of a variety of autoimmune rheumatic diseases. These molecules may be promising therapeutic targets for inflammatory arthritis. Recombinant human TNFRSF25, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

Human TL1A/TNFSF15 (CAT# ATGP1049) is coated at 10 µg/mL (100 ul/well) can bind Human DR3/TNFRSF25. The ED50 range ≤ 5 µg/mL.

Product Name Alternative

Tumor necrosis factor receptor superfamily member 25, Apo-3, Apoptosis-inducing receptor AIR, Apoptosis-mediating receptor DR3, Apoptosis-mediating receptor TRAMP, Death receptor 3, Lymphocyte-associated receptor of death, LARD, Protein WSL, Protein WSL-1, APO3, DDR3, DR3, TNFRSF12, WSL, WSL1, TR3

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.25 mg/mL (determined by absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human TL1A/TNFSF15 (CAT# ATGP1049) . The ED50 range ≤ 5 µg/mL.

Molecular Weight

46.1kDa (417aa)

Additionnal Information

ATGP3375, ATGP4017-10 µg, ATGP4017-20 µg, ATGP4017-50 µg, ATGP4017-100 µg, ATGP4017-250 µg, ATGP4017-500 µg, ATGP4017-1 mg, ATGP4017-10, ATGP4017-20, ATGP4017-50, ATGP4017-100, ATGP4017-250, ATGP4017-500, ATGP4017-1, ATGP4017-10 µg, ATGP4017-20 µg, ATGP4017-50 µg, ATGP4017-100 µg, ATGP4017-250 µg, ATGP4017-500 µg, ATGP4017-1 mg, ATGP4017-10, ATGP4017-20, ATGP4017-50, ATGP4017-100, ATGP4017-250, ATGP4017-500, ATGP4017-1

References & Citations

Slebioda TJ., et al. (2011) Eur J Immunol. 41:2606-2611.; ; Fang L., et al. (2008) J Exp Medl. 205:1037-1048.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Apoptosis

NCBI Accession Number

NP_003781.1

Uniprot Accession Number

Q93038

Species

Human

N Terminal Sequence

Ala

AA Sequence

QGGTRSP RCDCAGDFHK KIGLFCCRGC PAGHYLKAPC TEPCGNSTCL VCPQDTFLAW ENHHNSECAR CQACDEQASQ VALENCSAVA DTRCGCKPGW FVECQVSQCV SSSPFYCQPC LDCGALHRHT RLLCSRRDTD CGTCLPGFYE HGDGCVSCPT STLGSCPERC AAVCGWRQ