Recombinant human CD200 protein

CD200, also known as OX-2 membrane glycoprotein isoform a, belongs to the immunoglobulin superfamily. This protein imparts immune privileges by suppressing alloimmune and autoimmune responses through its receptor, CD200R, expressed primarily on myeloid cells. D200 and CD200R associate via their respective N-terminal Ig-like domains. CD200-CD200R signaling have been shown to play an important role in the regulation of anti-tumor immunity, and overexpression of CD200 has been reported in a number of malignancies, including CLL, as well as on cancer stem cells. Studies of the related genes in mouse and rat suggest that this gene may regulate myeloid cell activity and delivers an inhibitory signal for the macrophage lineage in diverse tissues. Elevated expression of this protein has been reported to be associated with poor prognosis in a number of human malignancies. In addition, it also plays an important role in prevention of graft rejection, autoimmune diseases and spontaneous abortion. Recombinant human CD200 protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Specifications

CD200 Subunit is coated at 10 µg/mL (100 ul/well) can bind Human CD200R1 (CAT# ATGP3809) in a Functional ELISA assay.

Product Name Alternative

OX-2 membrane glycoprotein isoform a, CD200, MOX1, MOX2, MRC, OX-2

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS-PAGE

Bioactivity

Measured by its binding ability in a functional ELISA with Human CD200R1 (CAT# ATGP3809)

Molecular Weight

23.5 kDa (211aa)

Additionnal Information

ATGP4013-10 µg, ATGP4013-20 µg, ATGP4013-50 µg, ATGP4013-100 µg, ATGP4013-250 µg, ATGP4013-500 µg, ATGP4013-1 mg, ATGP4013-10, ATGP4013-20, ATGP4013-50, ATGP4013-100, ATGP4013-250, ATGP4013-500, ATGP4013-1, ATGP4013-10 µg, ATGP4013-20 µg, ATGP4013-50 µg, ATGP4013-100 µg, ATGP4013-250 µg, ATGP4013-500 µg, ATGP4013-1 mg, ATGP4013-10, ATGP4013-20, ATGP4013-50, ATGP4013-100, ATGP4013-250, ATGP4013-500, ATGP4013-1

References & Citations

Akkaya M., et al. (2013) PLoS One. 8:e63325.; ; Li L., et al. (2016) Med Sci Monit. 22:1079-1084.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_005935

Uniprot Accession Number

P41217

Species

Human

N Terminal Sequence

Ala

AA Sequence

QVQVVTQ DEREQLYTPA SLKCSLQNAQ EALIVTWQKK KAVSPENMVT FSENHGVVIQ PAYKDKINIT QLGLQNSTIT FWNITLEDEG CYMCLFNTFG FGKISGTACL TVYVQPIVSL HYKFSEDHLN ITCSATARPA PMVFWKVPRS GIENSTVTLS HPNGTTSVTS ILHIKDPKNQ VGKEVICQVL HLGTVTDFKQ TVNKG