Recombinant human ULBP-3 protein

ULBP-3, also known as NKG2D ligand 3, is ligands for natural-killer group 2, member D (NKG2D) receptor. It is related to MHC class I molecules, but its gene maps outside the MHC locus. The domain structure of ULBP3 differs significantly from those of conventional MHC class I molecules. It does not contain the α3 domain and the transmembrane segment. ULBP3 is thus composed of only the α1α2 domain which is linked to the cell membrane by the GPI anchor. It functions as a stress-induced ligand for NKG2D receptor. In the normal hair follicle, ULBP3 is turned off. However, different studies reported its high level in alopecia areata (AA) . It is also expressed on some tumor cells and have been implicated in tumor surveillance. Recombinant human ULBP-3, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

NKG2D ligand 3, UL16 binding protein 3, N2DL-3, NKG2DL3, RAET1N, ALCAN-gamma, Retinoic acid early transcript 1N

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

1 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS - PAGE

Molecular Weight

49.3kDa (430aa)

Additionnal Information

ATGP4009-10 µg, ATGP4009-20 µg, ATGP4009-50 µg, ATGP4009-100 µg, ATGP4009-250 µg, ATGP4009-500 µg, ATGP4009-1 mg, ATGP4009-10, ATGP4009-20, ATGP4009-50, ATGP4009-100, ATGP4009-250, ATGP4009-500, ATGP4009-1, ATGP4009-10 µg, ATGP4009-20 µg, ATGP4009-50 µg, ATGP4009-100 µg, ATGP4009-250 µg, ATGP4009-500 µg, ATGP4009-1 mg, ATGP4009-10, ATGP4009-20, ATGP4009-50, ATGP4009-100, ATGP4009-250, ATGP4009-500, ATGP4009-1

References & Citations

Moftah NH, et al, (2016) Arch Dermatol Res. 308:415-421.; ; Wirsching HG, et al, (2019) JCI Insight. 4:e128217.

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_078794

Uniprot Accession Number

Q9BZM4

Species

Human

N Terminal Sequence

Ala

AA Sequence

DAHSLWY NFTIIHLPRH GQQWCEVQSQ VDQKNFLSYD CGSDKVLSMG HLEEQLYATD AWGKQLEMLR EVGQRLRLEL ADTELEDFTP SGPLTLQVRM SCECEADGYI RGSWQFSFDG RKFLLFDSNN RKWTVVHAGA RRMKEKWEKD SGLTTFFKMV SMRDCKSWLR DFLMHRKKRL EPTAPPTMAP G