Recombinant human CD6 protein

CD6, also known as T-cell differentiation antigen CD6, is a member of the group B SRCR (scavenger receptor cysteine-rich) superfamily. CD6 is a cell surface glycoprotein expressed primarily on T cells and It expressed by thymocytes, mature T-cells, a subset of B-cells known as B-1 cells, and by some cells in the brain. And T-cell differentiation antigen CD6 appears to play a role as both a co-stimulatory molecule in T cell activation and as an adhesion receptor. CD6 binds to CD166 (known as ALCAM), and is considered as a costimulatory molecule involved in lymphocyte activation and thymocyte development.. Certain alleles of this protein may be associated with susceptibility to multiple sclerosis. Recombinant human CD6, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

T-cell differentiation antigen CD6, T12, TP120, CD6, CD_antigen, T-cell differentiation antigen CD6 isform1

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE, Bioactivity

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

<1 EU per 1ug of protein (determined by LAL method)

Purity

90% by SDS - PAGE

Bioactivity

Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When cells are added to human CD6 coated plates 10 µg/mL. This effect is more to 50%.

Molecular Weight

68.3kDa (627aa)

Additionnal Information

ATGP4005-10 µg, ATGP4005-50 µg, ATGP4005-100 µg, ATGP4005-200ug, ATGP4005-250 µg, ATGP4005-500 µg, ATGP4005-1 mg, ATGP4005-10 µg, ATGP4005-20 µg, ATGP4005-50 µg, ATGP4005-100 µg, ATGP4005-250 µg, ATGP4005-500 µg, ATGP4005-1 mg, ATGP4005-10, ATGP4005-20, ATGP4005-50, ATGP4005-100, ATGP4005-250, ATGP4005-500, ATGP4005-1

References & Citations

Singer, N.G. et al. (1996) Immunology 88:537-547.; ; Namir J Hassan et al. (2004) Eur J Immunol. 34:930-940.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_006716

Uniprot Accession Number

P30203

Species

Human

N Terminal Sequence

Ala

AA Sequence

HPSPAPP DQLNTSSAES ELWEPGERLP VRLTNGSSSC SGTVEVRLEA SWEPACGALW DSRAAEAVCR ALGCGGAEAA SQLAPPTPEL PPPPAAGNTS VAANATLAGA PALLCSGAEW RLCEVVEHAC RSDGRRARVT CAENRALRLV DGGGACAGRV EMLEHGEWGS VCDDTWDLED AHVVCRQLGC GWAVQALPGL HFTPGRGPIH RDQVNCSGAE AYLWDCPGLP GQHYCGHKED AGAVCSEHQS WRLTGGADRC EGQVEVHFRG VWNTVCDSEW YPSEAKVLCQ SLGCGTAVER PKGLPHSLSG RMYYSCNGEE LTLSNCSWRF NNSNLCSQSL AARVLCSASR SLHNLSTPEV PASVQTVTIE SSVTVKIENK ESRELMLL