Recombinant human Carbohydrate sulfotransferase 3/CHST3 protein

CHST3, also known as chondroitin 6-O-sulfotransferase, is a member of sulfotransferase 1 family. The human CHST family is comprised of 14 enzymes and all members of this family are Golgi-localized type II membrane proteins. These enzymes utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the transfer of sulfate to position 6 of the N-acetylgalactosamine (GalNAc) residue of chondroitin. It can also sulfate Gal residues of keratan sulfate and Gal residues in sialyl N-acetyllactosamine (sialyl LacNAc) oligosaccharides. CHST3 Widely expressed in adult tissues. Expressed in heart, placenta, skeletal muscle and pancreas. Also expressed in various immune tissues such as spleen, lymph node, thymus and appendix. Recombinant human CHST3, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

C6ST, C6ST-1, C6ST1HSD, Carbohydrate (chondroitin 6) sulfotransferase 3, Chondroitin 6-O-sulfotransferase 1, Chondroitin 6-sulfotransferase, CHST-3, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0, GST-0, HSD

Expression System

Baculovirus

Antigen Species

Human

Tag

His-Tag

Applications

SDS-PAGE, Enzyme Activity

Concentration

0.25 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 90% by SDS - PAGE

Bioactivity

Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that sulfate from PAPS to Chondroitin Sulfate per minute at pH 7.5, at 25C.

Molecular Weight

51.3kDa (450aa)

Additionnal Information

C6ST, C6ST1, C6ST-1, C6ST1HSD, Carbohydrate (chondroitin 6) sulfotransferase 3, Carbohydrate sulfotransferase 3, Chondroitin 6 O sulfotransferase 1, Chondroitin 6 sulfotransferase, Chondroitin 6-O-sulfotransferase 1, Chondroitin 6-sulfotransferase, CHST 3, CHST3, CHST-3, Galactose/N-acetylglucosamine/N-acetylglucosamine 6 O sulfotransferase 0, Galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 0, GST0, GST-0, HSD, ATGP4001-10 µg, ATGP4001-20 µg, ATGP4001-50 µg, ATGP4001-100 µg, ATGP4001-250 µg, ATGP4001-500 µg, ATGP4001-1 mg, ATGP4001-010, ATGP4001-020, ATGP4001-050, ATGP4001-100, ATGP4001-250, ATGP4001-500, ATGP4001-01M

References & Citations

Hemmerich, S. and Rosen, S. Glycobiology 10:849-856.; ; Uchimura, K. et al. J. Biol. Chem.277:1443-1450.; ; Yusa, A. et al. J. Biol. Chem. 281:20393-20403.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Signal Transduction, Cytoskeleton

NCBI Accession Number

NP_004264

Uniprot Accession Number

Q7LGC8

Species

Human

N Terminal Sequence

Ala

AA Sequence

EKENKII SRVSDKLKQI PQALADANST DPALILAENA SLLSLSELDS AFSQLQSRLR NLSLQLGVEP AMEAAGEEEE EQRKEEEPPR PAVAGPRRHV LLMATTRTGS SFVGEFFNQQ GNIFYLFEPL WHIERTVSFE PGGANAAGSA LVYRDVLKQL FLCDLYVLEH FITPLPEDHL TQFMFRRGSS RSLCEDPVCT PFVKKVFEKY HCKNRRCGPL NVTLAAEACR RKEHMALKAV RIRQLEFLQP LAEDPRLDLR VIQLVRDPRA VLASRMVAFA GKYKTWKKWL DDEGQDGLRE EEVQRLRGNC ESIRLSAELG LRQPAWLRGR YMLVRYEDVA RGPLQKAREM YRFAGIPLTP QVEDWIQKNT QAAHDGSGIY STQKNSSEQF EKWRFSMPFK LAQVVQAACG PAMRLFGYKL ARDAAALTNR SVSLLEERGT FWVT