Recombinant rat Erythropoietin/EPO protein
EPO, also known as erythropoietin, is a member of t the type I cytokine family and is related to thrombopoietin. It is primarily produced in the kidney by a population of fibroblast-like cortical interstitial cells adjacent to the proximal tubules. This protein can be found in the plasma and regulates red cell production by promoting erythroid differentiation and initiating hemoglobin synthesis. It also has neuroprotective activity against a variety of potential brain injuries and anti-apoptotic functions in several tissue types. Recombinant rat Erythropoietin/EPO Protein, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Specifications
Specifications
Rat EPO stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED50 range ≤ 2 ng/mL.
Product Name Alternative
Erythropoietin, erythropoietin isoform 1 precursor, Epo
Expression System
HEK293
Antigen Species
Rat
Tag
His-Tag
Applications
SDS-PAGE, Bioactivity
Concentration
1 mg/mL (determined by Absorbance at 280 nm)
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Purity
> 95% by SDS - PAGE
Bioactivity
Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range ≤ 2 ng/mL.
Molecular Weight
19.6kDa (175aa)
Additionnal Information
, ATGP4000-10 µg, ATGP4000-20 µg, ATGP4000-50 µg, ATGP4000-100 µg, ATGP4000-250 µg, ATGP4000-500 µg, ATGP4000-1 mg
References & Citations
Koury, M.J., et al. (2005) Exp. Hematol. 33:1263.; ; Jelkmann W., et al. (2007) Eur J Haematol. 78:183-205.
Storage Conditions
Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Formulation
Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Scientific Category
Angiogenesis, Cardiovascular
NCBI Accession Number
NP_058697.1
Uniprot Accession Number
P29676
Species
Rat
N Terminal Sequence
Asp
AA Sequence
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