Recombinant human CD299/CLEC4M protein

CD299, as known as DC-SIGNR and CLEC4M, is a type II integral membrane protein that is 77% amino acid identical to CD209/DC-SIGN, an HIV gp120-binding protein. This protein is probable pathogen-recognition receptor involved in peripheral immune surveillance in liver. It may mediate the endocytosis of pathogens which are subsequently degraded in lysosomal compartments. CD299 is a receptor for ICAM3, binding to mannose-like carbohydrates. It also recognizes a wide range of evolutionarily divergent pathogens with a large impact on public health, including tuberculosis mycobacteria, and viruses including Ebola, hepatitis C, HIV-1, influenza A, West Nile virus and the SARS-CoV acute respiratory syndrome coronavirus. Recombinant human CD299, fused to hIgG-His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Specifications

Product Name Alternative

C-type lectin domain family 4 member M, CD209 antigen-like protein 1, DC-SIGN-related protein, DC-SIGNR, Dendritic cell-specific ICAM-3-grabbing non-integrin 2, DC-SIGN2, Liver/lymph node-specific ICAM-3-grabbing non-integrin, L-SIGN, CD209L, CD209L1

Expression System

Baculovirus

Antigen Species

Human

Tag

HIgG-His-Tag

Applications

SDS-PAGE

Concentration

0.5 mg/mL (determined by Absorbance at 280 nm)

Endotoxin

< 1 EU per 1ug of protein (determined by LAL method)

Purity

> 95% by SDS - PAGE

Molecular Weight

64.8kDa (570aa)

Additionnal Information

CLEC4M, ATGP3998-10 µg, ATGP3998-20 µg, ATGP3998-50 µg, ATGP3998-100 µg, ATGP3998-250 µg, ATGP3998-500 µg, ATGP3998-1 mg, ATGP3998-10, ATGP3998-20, ATGP3998-50, ATGP3998-100, ATGP3998-250, ATGP3998-500, ATGP3998-1

References & Citations

Gijzen K., et al, (2008) Exp Hematol. 36:860-870.; ; Johnson TR, et al, (2012) J Virol. 86:1339-1347.; ; Xia HB, et al, (2020) Oncol Lett. 19:69-76.;

Storage Conditions

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Formulation

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Scientific Category

Immunology

NCBI Accession Number

NP_055072

Uniprot Accession Number

Q9H2X3

Species

Human

N Terminal Sequence

Ala

AA Sequence

VSKVPSS LSQEQSEQDA IYQNLTQLKA AVGELSEKSK LQEIYQELTQ LKAAVGELPE KSKLQEIYQE LTRLKAAVGE LPEKSKLQEI YQELTRLKAA VGELPEKSKL QEIYQELTRL KAAVGELPEK SKLQEIYQEL TELKAAVGEL PEKSKLQEIY QELTQLKAAV GELPDQSKQQ QIYQELTDLK TAFERLCRHC PKDWTFFQGN CYFMSNSQRN WHDSVTACQE VRAQLVVIKT AEEQNFLQLQ TSRSNRFSWM GLSDLNQEGT WQWVDGSPLS PSFQRYWNSG EPNNSGNEDC AEFSGSGWND NRCDVDNYWI CKKPAACFRD E